DNA

Part:BBa_K5124003:Design

Designed by: Louise Brown   Group: iGEM24_Exeter   (2024-08-15)


Cas12a RD4_target_a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Researched them and found them present in the M.bovis genome.

Source

Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection.

References